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Cell biology

Cell viability

Cell viability is the proportion of cells in a sample that are alive and metabolically active at the time of testing, typically measured with a dye-exclusion or metabolic assay.

Viability testing methods vary by supplier and lot. Ask what method was used and what sample was tested before treating a reported percentage as comparable across products.

Viability can change between tissue recovery and final testing; freezing, thawing and storage conditions can all reduce the proportion of live cells. If viability data is provided, confirm it was measured on the specific lot or a matched sample, not assumed from the source tissue alone.

A 2026 analysis of six commercial WJ products found low cell viability with only rare viable MSCs. Viability data, when available, is one of the more direct ways to evaluate a cell-related claim on a tissue product; ask whether it was measured on the actual lot shipped rather than on a different reference sample.

Viability data, when available, is a more concrete basis for evaluating a cell-related claim than marketing language alone.

Sources

  1. Critical evaluation of compositions and clinical relevance of Wharton's jelly-derived biologics ↗PMC
  2. Colony forming potential and protein composition of commercial umbilical cord allograft products ↗American Journal of Sports Medicine